Influence of Salbutamol and Isoproterenol on the Production of TNF and Reactive Oxygen Species by Bovine Alveolar Macrophs and Calcitriol Differentiated HL-60 Cells

Abstract

The influence of the β-adrenergic agonists Salbutamol and Isoproterenol on the release of reactive oxyen species and Tumor Necrosis Factor (TNF) was tested with bovine alveolar macrophages and HL-60 cells differentiated to macrophages by calcitriol. The production of reactive oxygen species was analyzed by a microplate assay using dichlorofluorescein-diacetate. It could be shown that this method almost exclusively measures superoxide anions. TNF was determined by a bioassay with WEHI cells. The superoxide anion production was stimulated by Zymosan, the TNF release by LPS. By incubation with 5×10⁻⁶ and 5×10⁻⁷ M Salbutamol or 5×10⁻⁷ and 5×10⁻⁸ m lsoproterenol prior to the stimulation, the production of superoxide anions as well as of TNF was inhibited to a significant deree. The inhibitory effects of the adrenergic agonists were completely or at least partially inhibited by the respective antagonists, ICI 118.551 and Propanolol, respectively.