Plasma paraoxonase and arylesterase activities in smokers and smokeless tobacco users as Maraş powder

Abstract

Context: Maraş powder (MP), a different type of smokeless tobacco (ST) prepared from a tobacco called Nicotiana rustica Linn, is widely used in the Southern Turkey. Smoking and ST cause oxidative stress (OS) in the human body. Paraoxonase (PON) and arylesterase (ARE) are antioxidant enzymes.

Objective: To investigate the effects of MP on activities of PON, ARE, and malondialdehyde (MDA) levels in plasma and to compare these parameters in smokers and MP users (MPU).

Materials and methods: The study consisted of smokers, MPU, and control group (CG) neither smoking nor using MP healthy subjects. PON and ARE activities were measured spectrophotometrically using paraoxon and phenylacetate substrates, respectively.

Results: PON and ARE activities were decreased whereas MDA levels were increased in tobacco groups compared to the CG. The differences of ARE and MDA values between the tobacco groups and CG were found statistically significant (p < 0.01). But no significant differences were detected between the groups in the activity of PON (p > 0.05). However, the lowest activities of the enzymes were obtained in MPU.

Conclusion: Our results can help to evaluate harmful effects of cigarette and ST as MP. These effects can be attributed to increased OS. Increased plasma MDA levels and decreased ARE activities may be important in assessing oxidant/antioxidant imbalance in MPU as well as smokers. Also, using of MP has harmful effects at least cigarette smoking.

• Arylesterase
• Maraş powder
• Nicotiana rustica Linn
• oxidative stress
• paraoxonase
• smokeless tobacco