Localization of mRNA for Basic Fibroblast Growth Factor in Human Placenta

Abstract

The site of basic fibroblast growth factor (bFGF) gene expression in human placenta is not known. In situ hybridization using [³⁵S]-labelled riboprobe was employed to localize the sites of expression of bFGF mRNA in placental sections from early and late gestation. Autoradiographic hybridization signal was present in all placental tissue examined, with no significant hybridization with sense control. Basic FGF expression was seen in the syncytiotrophoblast surrounding the placental villi and in or around cytotrophoblast cells in first trimester placenta. At term, the bFGF gene expression was detected in the syncytiotrophoblast and fetal membranes. Strong expression of bFGF mRNA was localized in the smooth muscle cells around the mid and large sized placental vessels. The detection of bFGF mRNA in the placenta during early pregnancy suggests a role for bFGF in angiogenesis, whereas, its expression at term suggests that bFGF may be associated with differentiated functions of the trophoblast.

Key Words:

• human placenta
• trophoblast
• amnion
• basic FGF and in situ hybridization