Receptor Binding and Biological Activity of Mammalian Expressed Sensory and Motor Neuron-derived Factor (SMDF)

Abstract

Sensory and motor neuron-derived factor (SMDF) is a member of the neuregulin family of proteins. SMDF is structurally characterized by a novel N-terminal domain. Using the signal sequence and N-terminal 28 amino acids (the “epitope”) of herpes simplex virus type 1 glycoprotein D (gD), we have expressed SMDF as an epitope-tagged protein (gD-SMDF) in 293 cells, and purified it to > 98% homogeneity on a monoclonal anti-gD column. gD-SMDF stimulates human Schwann cell growth and ³H-thymidine incorporation in MCF-7 and T47D human breast tumor cells in vitro The biological activity of gD-SMDF is consistent with its ability to compete with ¹²⁵I-labeled heregulinβ1 peptide (rHRGβ1 _177-244) to bind to soluble dimeric ErbB receptor-IgG fusion proteins. gD-SMDF binds with low affinity to homodimeric ErbB3-IgG and ErbB4-IgG but with higher affinity to hetero-dimeric ErbB2/ErbB3-IgG and ErbB2/ErbB4-IgG. Using a SMDF-IgG(Fc) fusion protein we generated a monoclonal antibody (3G11) which binds SMDF, crossreacts with rHRGβ1 _177-244 and neutralizes the in vitro activities of gD-SMDF and rHRGβ1 _177-244 in human Schwann cells.

Key Words:

• SMDF
• neuregulins
• Schwann cells
• ErbB receptors