Differential expression of poliovirus receptor, regulator of G-protein signaling 11 and erythrocyte protein band 4.1-like 3 in human granulosa cells during follicular growth and maturation

Abstract

Poliovirus receptor (PVR), regulator of G-protein signaling-11 (RGS11), and erythrocyte protein band-4.1-like 3 (EPB41L3) have been proposed to function in follicular maturation in mouse models. We have examined their expression in human mural (mGCs) and cumulus granulosa cells (CCs). Expression of PVR and RGS11 in mGCs decreased in medium-sized follicles compared to small follicles of IVM cycles and increased again in large follicles. Luteinization caused decreased expression of both PVR and RGS11. In vitro incubation of mGCs with progesterone-rich conditioned media decreased expression of RGS11 without affecting PVR levels. Inhibition of progesterone signaling enhanced expression of both RGS11 and PVR. Expression in CCs was examined by means of global transcriptome sequencing analysis RGS11 and EPB41L3 increased in CCs during follicular maturation while PVR levels did not change. In conclusion, during human follicular maturation there are significant changes in expression of PVR, RGS11 and EPB41L3, possibly regulated by progesterone.

Chinese abstract

既往已经证明在小鼠模型中，卵泡的成熟过程中可能有脊髓灰质炎病毒受体（Poliovirus receptor，PVR），G-蛋白信号-11调控子（G-protein signaling-11，RGS11），以及红细胞蛋白质带-4.1-3在发挥作用。在人类卵泡壁细胞（mGCs）以及卵丘颗粒细胞（CCs）中，我们已经发现有这些蛋白表达。IVM周期中，与小卵泡相比，中等大小卵泡mGCs中PVR以及RGS11表达下降，但是在大卵泡mGCs中表达再次增多。黄素化导致PVR以及RGS11的表达均下降。试管孵化mGCs过程中，富含孕激素的培养液使得其中RGS11表达下降，但是不影响PVR的表达情况。阻断孕激素信号通路使得RGS11以及PVR的表达上调。在卵泡成熟过程中，应用全球转录酶测序分析法发现RGS11以及EPB41L3在CCs中的表达上调，但是PVR的表达没有改变。因此得出结论，在人类卵泡成熟过程中PVR，RGS11以及EPB41L3的表达发生改变，可能这些改变都是由孕激素调节的。

• Erythrocyte protein band-4.1-like 3
• follicular growth
• granulosa
• poliovirus receptor
• regulator of G-protein signaling-11