Increased levels of oxidative and carbonyl stress markers in normal ovarian cortex surrounding endometriotic cysts

Abstract

Many evidence support the view that endometriotic cyst may exert detrimental effect on the surrounding ovarian microenvironment so representing a risk to functionality of adjacent follicles. Patients with benign ovarian cyst (endometriotic, follicular and dermoid cysts) subjected to laparoscopic cystectomy were enrolled in the present retrospective study in order to analyze whether endometriotic tissue could negatively affect the surrounding normal ovarian cortex more severely than other ovarian cysts. To this end we carried out immunohistochemistry analysis and comparative determination of the transcription factor FOXO3A, oxidized DNA adduct 8-OHdG (8-hydroxy-2′-deoxyguanosine) and damaged proteins known as AGEs (Advanced Glycation End products) as markers of ovarian stress response and molecular damage. Our results show that all the markers analyzed were present in normal ovarian tissue surrounding benign cysts. We observed higher levels of FOXO3A (15.90 ± 0.28), 8-OHdG (13.33 ± 2.07) and AGEs (12.58 ± 4.34) staining in normal ovarian cortex surrounding endometriotic cysts in comparison with follicular cysts (9.04 ± 0.29, 2.67 ± 2.67, 11.31 ± 2.95, respectively) and dermoid cysts (2.02 ± 0.18, 4.33 ± 2.58 and 10.56 ± 4.03, respectively). These results provide evidence that ovarian endometrioma is responsible for more severe alterations to cellular biomolecules than follicular and dermoid cysts.

Chinese abstract

许多证据支持这一观点：子宫内膜异位囊肿可能对卵巢周边微环境产生不利影响，由此代表对相邻卵泡功能的危害。良性卵巢囊肿患者（子宫内膜异位，滤泡和皮样囊肿）经腹腔镜囊肿切除术后参加了回顾性研究，是为了分析是否异位内膜组织比其他卵巢囊肿对周围正常卵巢皮质可产生更严重的不利影响。为此我们进行了免疫组化分析和转录因子FOXO3A的比较测定，氧化DNA加合物8-羟基脱氧鸟苷（8-hydroxy-20-deoxyguanosine）和受损的蛋白质被称为AGEs（晚期糖基化终末产物）作为卵巢应激反应和分子损伤的标记。我们的研究结果表明，所有分析的标记都存在于正常卵巢周围组织的良性囊肿中。我们在正常卵巢皮质周围的子宫内膜异位囊肿中观察到的更高水平的FOXO3A（15.90±0.28），8-羟基脱氧鸟苷（13.33±2.07）和AGEs（12.58±4.34）染色，相比滤泡囊肿（分别为9.04±0.29，2.67±2.67，11.31±2.95）及皮样囊肿（分别为2.02±0.18，4.33，2.58和10.56±±4.03）。这些结果提供的证据表明，卵巢子宫内膜异位囊肿比卵泡和皮样囊肿能更明显的改变细胞生物分子。

• 8-OHdG
• advanced glycation endproducts
• endometriosis
• FOXO3A
• methylglyoxal
• ovarian cyst
• oxidative stress

Notice of Correction

The version of this article published online ahead of print on 17 Jul 2014 contained an error in the author details. Author “Adina Di Fonso” was mistakenly listed as “Adina Di Fonso Testo”. The error has been corrected for this version.