Rapid peptidomic profiling of peritoneal fluid by MALDI-TOF mass spectrometry for the identification of biomarkers of endometriosis

Abstract

Peptidomic profiling of peritoneal fluid by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF-MS) may represent a promising, suitable, rapid method for early diagnosis and staging of endometriosis. In a case-control study, peritoneal fluid was collected from 23 patients affected by endometriosis (eight minimal/mild endometriosis and 15 moderate/severe endometriosis) and six “endometriosis free” women undergoing laparoscopy. MALDI-TOF mass spectra of the peptide fraction extracted from peritoneal fluid samples lead to identify biomarkers potentially suitable for discriminating between peritoneal fluid samples from women affected by minimal/mild endometriosis and those from women affected by moderate/severe endometriosis. Peptidomic analysis of peritoneal fluid samples may define putative peptide biomarkers suitable for staging endometriosis and improve our understanding of the pathogenesis of endometriosis.

Chinese abstract

通过基质辅助激光角吸附电离飞行时间质谱（MALDI-TOF-MS）进行腹膜液多肽组学测定也许可为子宫内膜异位症的早期诊断与分期提供一个可观、适用、快速的方法。在一项病例对照研究中，腹腔镜下采集了23位子宫内膜异位症患者的腹膜液（8位极轻/轻度患者，15位中度/重度患者）与6位非子宫内膜异位症女性的腹膜液。对提取自腹膜液样本的肽片段通过MALDI-TOF质谱分析测定生物标志物，可鉴别腹膜液样本是来自极轻/轻度子宫内膜异位症患者还是来自中度/重度患者。腹膜液样本的肽测定也许证实了肽生物标志物适用于子宫内膜异位症分期这一推断，且促进了我们对子宫内膜异位症发病机制的了解。

• Biomarkers
• endometriosis
• laparoscopy
• MALDI-TOF-MS
• peptidomics

Supplementary material available online

Supplemental Digital Content (S1, S2).