Anti-aging effect of estrogen on telomerase activity in ovariectomised rats – animal model for menopause

Abstract

Objective: The aim of this study was to investigate the anti-aging effects of exogenous estrogen on telomerase activity in ovariectomized female Sprague-Dawley rats.

Methods: Thirty-three 12-week-old female rats were divided into three groups: the ovariectomized-Treated group (Treated, n = 11), the ovariectomized control group (OVX, n = 11) and the Sham-operated group (Sham, n = 11). The rats in the Treated group were given 0.21 mg/kg estradiol valerate intragastric administration while other two groups were given the amount of physiological saline daily. All of the animals were euthanized 12 weeks after treatment, and abdominal aortic blood samples were taken to assess the level of estradiol (E₂), follicle stimulating hormone (FSH). Telomerase activity and telomerase reverse transcriptase (TERT) mRNA expression in the heart, liver, brain tissues of all rats were measured by reverse transcriptional polymerase chain reaction (RT-PCR) and competitive enzyme-linked immunosorbent assay (ELISA).

Results: Compared to the OVX and Sham group, telomerase activity and TERT mRNA levels were significantly increased in the heart, liver and brain tissues of rats in the Treated group (p < 0.05). The telomerase expression was significantly higher in the heart than in liver and brain tissues in the Treated group (p < 0.05).

Conclusion: Based on the findings in the ovariectomized female rats model, exogenous estrogen can significantly up-regulate telomerase activity and TERT mRNA expression to exert the effects of anti-aging.

• Aging
• estrogen
• menopause
• telomerase
• telomere

Acknowledgements

We thank all the subjects for their participation in this research. We also thank the co-workers from the department of Central Laboratory, for their support to the MENOPAUSE project.

Declaration of interest

The present study was funded by the Science and Technology Department of Zhejiang Province, Hangzhou, China (WKJ-ZJ-024 and 2014C03044-1) and Hangzhou Municipal Science and Technology Bureau, Hangzhou, China (20142013A58). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.