Specific activation, signalling and secretion profiles of human platelets following PAR-1 and PAR-4 stimulation

Abstract

Blood platelets play a central haemostatic function; however, they also play a role in inflammation and are capable of secreting various cytokines, chemokines and related products. The purpose of this study was to identify subtle variations in platelet physiology using proteomics. We compared the levels of membrane proteins (n = 3), α and δ granule proteins (n = 18), and signalling proteins (n = 30) from unstimulated platelets with those of protease-activated receptor (PAR)-1- and PAR-4-stimulated platelets (n = 10). The vast majority of these proteins responded similarly to PAR-1 or PAR-4 engagement. However, differences were observed within membrane CD40L expressed, and α granule GRO-α and MDC secreted proteins.

• Immunologic factors
• inflammation
• platelet
• protease-activated receptors
• signal transduction

Acknowledgements

We thank Drs Catherine Argaud, Alain Lefebvre and Patricia Chavarin, and Ms Marie-Ange Eyraud and Mr Charles-Antoine Arthaud (EFS Auvergne-Loire, France) for their help in obtaining and preparing the human blood cells.

Declaration of interest

The authors declare no competing financial interests and no conflicts of interest regarding this study. This study was supported by the Etablissement Français du Sang – EFS, the French National Agency for Drug Safety and Health Products (ANSM – AAP-2012-011), the “Amis de Rémi” Association, and Agence Nationale de la Recherche (ANR), reference ANR-12-JSV1-0012-01.

Supplementary material available online.