Abstract
Purpose: To investigate the effects of gamma pre-exposure on cadmium accumulation in Plantago ovata seedlings. Metallothionein (MT) localization was also studied following Cadmium (Cd) treatment in P. ovata.
Materials and methods: DNA damage was determined by alkaline comet assay. MT gene and protein expression were studied by real-time polymerase chain reaction and flow cytometry, respectively, in root and shoot tissues. Metal accumulation (Cd, zinc [Zn], iron [Fe]) was evaluated by Atomic Absorption Spectroscopy.
Results: Cd treatment decreased seed germination rate, biomass and free radical scavenging activity and increased DNA damage in a dose-dependent manner. When P. ovata seeds were pre- exposed to 5 Gy gamma dose (prior to Cd treatment) seed germination rate, biomass and free radical scavenging activity increased significantly. MT genes (PoMT1, PoMT2 and PoMT3) and MT protein expression enhanced when 5 Gy gamma-irradiated seeds were grown in Cd containing medium and Cd accumulation also increased in a dose-dependent manner.
Conclusions: Higher Cd accumulation in P. ovata seedlings may be attributed to the upregulation of PoMT genes in gamma pretreated seedlings. Localization of metallothionein in cytosol and nucleus indicated its positive role against Cd-mediated cytotoxic and genotoxic effects.
Acknowledgements
The authors would like to thank Dr Avijit Saha, UGC-DAE Consortium for Scientific Research, Kolkata Centre, India, for providing the cobalt-60 (60Co) gamma irradiation facility and Salvaraj Subburaj of the above centre for his invaluable assistance in fluorescence microscopy imaging. They express sincere gratitude to Dr Sanjaya Mallick (Application Scientist, BD Bioscience, CRNN, Kolkata) for confocal microscopy imaging and FACS analysis. The first author is grateful to UGC-DAE-CSR for financial assistance (Grant no: UGC-DAE-CSR/KC/CRS/09/RB-04/1337 dt 31/07/2009).
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.