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Abstract
Purpose To evaluate the effect of oxygen pressure during incubation with a 10B-carrier on 10B uptake capacity of cultured p53 wild-type and mutated tumor cells.
Materials and methods Cultured human head and neck squamous cell carcinoma cell line transfected with mutant TP53 (SAS/mp53), or with a neo vector as a control (SAS/neo) was incubated with L-para-boronophenylalanine-10B (BPA) or sodium mercaptoundecahydrododecaborate-10B (BSH) as a 10B-carrier at the 10B concentration of 60 ppm for 24 h under aerobic (20.7% of oxygen) or hypoxic (0.28% of oxygen) conditions. Immediately after incubation, cultured tumor cells received reactor thermal neutron beams, and a cell survival assay was performed. 10B concentration of cultured SAS/neo or SAS/mp53 cells incubated under aerobic or hypoxic conditions was determined with a thermal neutron guide tube.
Results Hypoxic incubation significantly decreased 10B concentration of cultured cells with a clearer tendency observed following BPA than BSH treatment in both SAS/neo and SAS/mp53 cells. Following neutron beam irradiation, SAS/mp53 cells showed significantly higher relative biological effectiveness values than SAS/neo cells because of the significantly lower radiosensitivity of SAS/mp53 to γ-rays than SAS/neo cells.
Conclusion Oxygen pressure during incubation with a 10B-carrier had a critical impact on 10B uptake of cultured tumor cells.
Acknowledgements
This study was supported, in part, by a Grant-in-aid for Challenging Exploratory Research (26670556) and a Grant-in-aid for Scientific Research (B) (15H04295) from the Japan Society for the Promotion of Science.
Declaration of interest
The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.