Chelating, antioxidant and hypoglycaemic potential of Muscari comosum (L.) Mill. bulb extracts

Abstract

The metal chelating activity, antioxidant properties and the effect on carbohydrate-hydrolysing enzyme inhibition of Muscari comosum extracts have been investigated. M. comosum bulbs contain a total amount of the phenols with a value of 56.6 mg chlorogenic acid equivalent per gram of extract and a flavonoid content of 23.4 mg quercetin equivalent per gram of extract. In order to evaluate the non-polar constituents, n-hexane extract was obtained. Gas chromatography–mass spectrometry analysis revealed the presence of fatty acids and ethyl esters as major constituents, with different aldehydes and alkanes as minor components. Ethanolic extract had the highest ferric-reducing ability power (66.7 μM Fe(II)/g) and DPPH scavenging activity with a concentration giving 50% inhibition (IC₅₀) value of 40.9 μg/ml. Moreover, this extract exhibited a good hypoglycaemic activity with IC₅₀ values of 81.3 and 112.8 μg/ml for α-amylase and α-glucosidase, respectively. In conclusion, M. comosum bulbs show promising antioxidant and hypoglycaemic activity via the inhibition of carbohydrate digestive enzymes. These activities may be of interest from a functional point of view and for the revalorization of this ancient non-cultivated vegetable of Mediterranean traditional gastronomy.

Keywords::

• Muscari comosum
• bulbs
• antioxidant activity
• chelating activity
• hypoglycaemic activity
• total phenolic and flavonoid content
• lipophilic profile

Acknowledgements

The authors with to thank Dr N. Passalacqua, Natural History Museum of Calabria and Botanic Garden, University of Calabria, Italy, for identify the samples used in this study.

Declaration of interest: The authors report no conflicts of interest. The study was not specifically funded. The authors alone are responsible for the content and writing of the paper.