The chromatographic analysis of oligosaccharides and preparation of 1-kestose and nystose in yacon

Abstract

The thin-layer chromatographic analysis of the crude oligosaccharides extracted from yacon revealed the presence of glucose, fructose, sucrose, 1-kestose and nystose. The qualitative and quantitative analysis was carried out on oligosaccharides by high pressure liquid chromatography and the results showed that the contents of d-glucose, fructose, sucrose, 1-kestose, nystose and 1-fructofuranosyl nystose in oligosaccharides were 38.30%, 16.44%, 14.58%, 12.29%, 12.17%, 6.20%, respectively. The content of the fructooligosaccharides in oligosaccharides was 30.66%. The crude oligosaccharides were separated and purified by silica gel column chromatography. The two fractions obtained from crude oligosaccharides were 1-kestose and nystose, which were identified by mass spectra. The yield of 1-kestose and nystose were 10.36% and 9.73%, respectively. The purity of 1-kestose was 82.9% and of nystose was 73.6%.

Keywords::

• yacon
• oligosaccharide
• analysis
• separation

Declarations of interest: This work was financially supported by National Agricultural Innovation Project (No. 2011GB2A100009), Natural Science Foundation of Tianjin City (Nos. 09JCZDJC21800 and 09JCYBJC15800), National Natural Science Foundation of China (NSFC, No. 31000279), the Scientific and Technical Supporting Programs of Science and Technology Commission Foundation of Tianjin (No. 08ZCGHHZ00800), the Foundation of Tianjin Educational Committee (Nos. 20090604) and Recruited faculty research start-up foundation in Tianjin University of Science and Technology (Nos. 0200060). The authors alone are responsible for the content and writing of the paper.