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Abstract
Several recent reports describe a role of probiotics as a therapeutic approach for celiac disease (CD). Two undigested A-gliadin peptides, P31-43 and P57-68, are central to CD pathogenesis, inducing an innate and an adaptive immune response, respectively. They enter enterocytes and localize to vesicular compartment to induce their toxic/immunogenics effects. In this article, we tested the effect of probiotic Lactobacillus paracasei (LP) CBA L74 (International Depository Accession Number LMG P-24778), its supernatant and LP-fermented cereals on gliadin peptides, P31-43 and P57-68, entrance in Caco-2 cells. Both LP CBA L74 and its supernatant inhibit P31-43 (intensity of fluorescence; FI: 75%) and P57-68 (FI: 50%) entrance in Caco2 cells, indicating that this biological effect is due to some product included in LP CBA L74 supernatant. This effect was present also after fermentation of cereals. This study describes a novel effect of probiotics in the prevention of undigested gliadin peptides toxic effects.
Author’s contributions
Marco Sarno, MD, performed the experiments and collected, analyzed and interpreted the data; Giuliana Lania, PhD, analyzed and interpreted the data; Marialaura Cuomo, MD, provided statistical analysis; Dr. Federica Nigro and Dr. Francesca Passannanti provided all of the supernatant for the study; Dr. Andrea Budelli and Dr. Francesca Fasano provided LP CBA L74 and fermented cereals; Prof. Riccardo Troncone and Prof. Salvatore Auricchio critical reading of the manuscript; Prof. Maria Vittoria Barone is the principle investigator for this study providing oversight and contributed fundamental conceptualization for the research, writing the manuscript; Prof. Roberto Nigro provided LP CBA L74 and fermented cereals and contributed fundamental conceptualization for the research; and Merlin Nanayakkara, PhD, performed the experiments and collected, analyzed and interpreted the data.
Supplementary material available online Supplementary Figures 1 and 2