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Biocatalysis and Biotransformation Volume 33, 2015 - Issue 3
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Original Article

Ring-opening polymerization of ɛ-caprolactone catalyzed by a novel lipase Candida sp. 99-125

Wei HeCollege of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Technology University, Nanjing, China and
,
Zheng FangSchool of Pharmaceutical Science, Nanjing Technology University, Nanjing, China
,
Ning ZhuCollege of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Technology University, Nanjing, China and
,
Dong JiCollege of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Technology University, Nanjing, China and
,
Zhenjiang LiCollege of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Technology University, Nanjing, China and
&
Kai GuoCollege of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Technology University, Nanjing, China and Correspondence[email protected]
Pages 150-155 | Received 26 Nov 2014, Accepted 31 Aug 2015, Published online: 28 Sep 2015
 
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Abstract

The ring-opening polymerization of ɛ-caprolactone catalyzed by a novel lipase Candida sp. 99-125 was achieved in organic solvents. ɛ-Caprolactone was found to be polymerized in quantitative yield in toluene at 80 °C for 5 days. The poly (ɛ-caprolactone) obtained had a number-average molecular weight of 14,120 (polydispersity index = 1.161). The effect of enzyme concentration, temperature, reaction time, reaction medium, and water content on monomer conversion and product molecular weight were investigated. Kinetic analysis showed that the enzyme displayed Michaelis–Menten kinetics and showed stronger affinity for ɛ-caprolactone (with a Km value of 6.1 mmol/L) than other reported lipases.

Declaration of interest

The research has been supported by National Key Basic Research Program of China (973 Program) 2012CB725204 and 2011CB710803, a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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