Activation of human leukemic mast cell line LAD2 is modulated by dehydroleucodine and xanthatin

Abstract

The aim of the present study was to determine whether dehydroleucodine, xanthatin and 3-benzyloxymethyl-5H-furan-2-one inhibit the activation of human leukemic LAD2 mast cells induced by compound 48/80 or the calcium ionophore A23187. LAD2 cells were preincubated in the presence of test drugs and then challenged with the secretagogues. This study provides the first evidence in favor of the view that dehydroleucodine and xanthatin inhibit the degranulation of LAD2 cells, thus acting as human mast cell stabilizers. These molecules could be effective in the treatment of human diseases associated with inappropriate mast cell activation.

Keywords::

• LAD2 mast cells
• dehydroleucodine
• xanthatin
• 3-benzyloxymethyl-5H-furan-2-one
• compound 48/80
• A23187

Acknowledgements

We thank Dr. Juan Carlos de Rosas and Dr. Gina Rojas for their technical advice. This work was supported by grants from the Consejo Nacional de Investigaciones Científicas y Técnicas (PIP-CONICET 5128) and Secretaría de Ciencia, Técnica y Postgrado de la Universidad Nacional de Cuyo (SeCTyP UNCuyo 06/J268), Argentina.

Potential conflict of interest

Disclosure forms provided by the authors are available with the full text of this article at www.informahealthcare.com/lal.