Comparison of different DNA extraction methods from peripheral blood cells: advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network

Abstract

Genomic DNA extraction is a primary component of genomic research and diagnostic routine analysis. Recently, the importance of this process has been highlighted by the necessity to standardize the diagnostic procedure. In this regard, the Minimal Residual Disease (MRD) Network of the Fondazione Italiana Linfomi (FIL MRD Network) has performed a comparative study of four different commercially available kits for DNA extraction, applying them on a panel of cellular pellets, with the aim of defining possible technical recommendations in order to harmonize and standardize diagnostic procedures in the clinical setting. Overall, all four kits usually allowed the recovery of a significant quantity of high-quality DNA (in most conditions), although specific indications could be addressed for cellular pellets of different sizes.

Keywords::

• DNA extraction
• FIL MRD Network
• PCR

Potential conflict of interest

Disclosure forms provided by the authors are available with the full text of this article at www.informahealthcare.com/lal.

This work was supported by the Centro Interdipartimentale per la Ricerca sul Cancro “G. Prodi”, BolognAIL, AIRC (IG10519; 10007 5 × Mille; IG 2013 N.14355), RFO (Prof. Pileri and Prof. Piccaluga), Progetto Strategico di Ateneo 2006 (Prof. Pileri and Prof. Piccaluga) and FIRB Futura 2011 RBFR12D1CB (Prof. Piccaluga).