A simple and rapid nuclear staining method for Rhizoctonia solani Kuhn

Abstract

We developed a modified staining technique using acridine orange to stain the nuclei of Rhizoctonia solani. Acridine orange solution was prepared in acetic acid buffer, pH 7.2. Staining for 15 min was critical for observing the nuclei. All of the isolates were found to be multinucleated. The nuclei appeared bright green with light orange background. This method is simple, rapid and reproducible.

Key words::

• acridine orange
• nuclear staining
• Rhizoctonia solani

Acknowledgment

The authors thank Yuvaraja's College, University of Mysore, JSS College, BN Road, Mysore, Karnataka, India for use of the facilities.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.