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Stain Technology Volume 28, 1953 - Issue 2
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Original Article

Softening Paraffin-Embedded Plant Tissues

Stanley M. Alcorn Department of Plant Pathology, University of California, Berkeley, California
&
Peter A. Ark Department of Plant Pathology, University of California, Berkeley, California
Pages 55-56 | Received 06 Oct 1952, Published online: 12 Jul 2009
 
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Abstract

Soaking paraffin-embedded plant specimens 2–3 days at 37°C. in a mixture of glycerol, 10 ml., Dreft, 1 g., and water 90 ml. is an effective means of softening them prior to sectioning. One side of the paraffin block must be pared away to expose the tissue before immersion in the softening solution.

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