Staining Processed Radioautographs

Abstract

Integrated radioautographs obtained by coating with melted nuclear emulsion (Eastman's NTB3) are serially processed and stained at 4° C. Kingsley's mixture of methylene blue-azure A at pH 6.9 is followed by 0.05% NaHSO₃, which destains the emulsion. Basic fuchsin, 0.05% aqueous, is used as counterstain, followed by dehydration in absolute ethanol, clearing in cedarwood oil and mounting with Canada balsam. Basic fuchsin alone produces an oftentimes satisfactory monochromatic background.