Modifications of a Method for Preparing Retinal Wholemounts for Sectioning

Abstract

A method for embedding and sectioning retinal wholemounts is described. It employs a hydrophilic embedding agent, LR White, and coverslips inert to standard embedding resins. This simple technique, which has worked successfully on both enzymatically and autoradiographically labelled material, provides a means by which retinal wholemounts can be cut nearly parallel to the plane of the retinal layers. Previously prepared retinal whole-mounts can also be successfully embedded and sectioned more than a year later by the method described.