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Biotechnic & Histochemistry Volume 71, 1996 - Issue 2
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Original Article

Differential Staining for Cellulosic and Modified Plant Cell Walls

Bruno Mori Department of Plant Biology, University of Firenze, P. le Cascine 28, 50144, Firenze, Italy
&
Lorenza M. Bellani Department of Plant Biology, University of Firenze, P. le Cascine 28, 50144, Firenze, Italy; Institute of General Biology, University of Siena, Via T. Pendola 62, 53100, Siena, Italy
Pages 71-72 | Published online: 12 Jul 2009
 
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Abstract

A simple method to enhance the staining of cell wall components for fluorescence microscopy is described. In stems of Nicotiana tabacum and needles of Pinus eldarica lignin, the cuticle and unsaturated lipids are indicated by a purple-red fluorescence while pectocellulosic components fluorescc pale blue.

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