Staining Paraffin Embedded Sections of Scald of Barley before Paraffin Removal

Abstract

Staining of paraffin embedded sections with periodic acid-Schiff reagent and fast green before paraffin removal resulted in differentiation of barley seed and leaf tissue from fungal structures of Rhynchosporium secalis. Crystal violet, toluidine blue O and aniline blue also successfully stained fungal structures of R. secalis in barley leaf tissues. Staining of embedded sections before paraffin removal allows simple processing of a series of sections, saves time and reduces solvent consumption.

Key Words:

• leaf infection
• paraffin embedment
• PAS reagent
• Rhynchosporium secalis
• scald of barley
• seed infection