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Original Article

Collagen nanofiber containing silver nanoparticles for improved wound-healing applications

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Pages 520-529 | Received 23 May 2015, Accepted 15 Sep 2015, Published online: 20 Oct 2015
 

Abstract

Electrospun nanofibers showing great promise for fabricating nanostructured materials might help to improve the quality of wound care. The present study aimed to investigate the wound-healing potential of collagen nanofiber mats containing silver nanoparticles. Silver nanoparticles (AgNPs) synthesized by the chemical reduction method were incorporated in collagen nanofibers during the electrospinning process. Characterization of electrospun nanofiber mats revealed a mean fiber diameters in the range of 300–700 nm with a sustained release of silver ions shown to follow pseudo-order kinetics. MIC of AgNPs against Staphylococcus aureus and Pseudomonas aeruginosa were evaluated using micro-dilution assay and further antimicrobial activity of fabricated nanofibers was performed. Finally, in vivo studies were performed to demonstrate the wound-healing efficacy of composite nanofibers. In vitro results confirmed the potential antimicrobial efficacy provided by AgNPs and AgNPs composite nanofibers, essential to provide an aseptic environment at the wound site. In vivo study revealed that the rate of wound healing of the composite nanofiber mats was found to be accelerated compared with plain collagen nanofibers. Histology analysis revealed an accelerated re-epithelization, collagen production, and better wound contraction with AgNPs composite collagen nanofibers.

Declaration of interest

The authors Dr. Goutam Rath and Dr. Amit K Goyal are thankful to the Department of Biotechnology (DBT), New Delhi (F. No. 102/IFD/SAN/2577/2011–2012 dated September 28, 2011) and Punjab Technical University, Kapurthala, Punjab, for providing financial assistance to carry out research.

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