Analysis of association of clinical aspects and IL1B tagSNPs with severe preeclampsia

ABSTRACT

Objective: This study investigates the association between IL1B genotypes using a tag SNP (single polymorphism) approach, maternal and environmental factors in Brazilian women with severe preeclampsia. Methods: A case-control study with a total of 456 patients (169 preeclamptic women and 287 controls) was conducted in the two reference maternity hospitals of Sergipe state, Northeast Brazil. A questionnaire was administered and DNA was extracted to genotype the population for four tag SNPs of the IL1Beta: rs 1143643, rs 1143633, rs 1143634 and rs 1143630. Haplotype association analysis and p-values were calculated using the THESIAS test. Odds ratio (OR) estimation, confidence interval (CI) and multivariate logistic regression were performed. Results: High pregestational body mass index (pre-BMI), first gestation, cesarean section, more than six medical visits, low level of consciousness on admission and TC and TT genotype in rs1143630 of IL1Beta showed association with the preeclamptic group in univariate analysis. After multivariate logistic regression pre-BMI, first gestation and low level of consciousness on admission remained associated. Conclusion: We identified an association between clinical variables and preeclampsia. Univariate analysis suggested that inflammatory process-related genes, such as IL1B, may be involved and should be targeted in further studies. The identification of the genetic background involved in preeclampsia host response modulation is mandatory in order to understand the preeclampsia process.

KEYWORDS:

• Case-control study
• maternal morbidity and mortality
• molecular biology
• polymorphisms
• preeclampsia

Funding

This project was funded by personal resources first and by the project “Avaliation of genotype and phenotype influence by infectious agents and host in the pathogenesis of infectious and parasitic diseases” of PRONEX 2009 of FAPITEC.

The Scientific Editing Company Ltd. provided the English language editing service.

Additional information

Funding

This project was funded by personal resources first and by the project “Avaliation of genotype and phenotype influence by infectious agents and host in the pathogenesis of infectious and parasitic diseases” of PRONEX 2009 of FAPITEC.

Notes on contributors

Larissa Paes Leme Galvão

LPG and RQG designed the study and participated in all phases of the study. FFM collected data and LPG supervised it. FAP and IDCB performed the statistical analysis. FAP and CKAP were the responsible for the DNA extraction and analysis. All authors read, reviewed and agreed with the final version.

Ricardo Gurgel

LPG and RQG designed the study and participated in all phases of the study. FFM collected data and LPG supervised it. FAP and IDCB performed the statistical analysis. FAP and CKAP were the responsible for the DNA extraction and analysis. All authors read, reviewed and agreed with the final version.