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ABSTRACT
Objective: To analyze methylation profiles of known preeclampsia/eclampsia (PE) candidate genes in normal (NL) and preeclamptic (PE) women at delivery. Methods: A matched case–control study comparing methylation in 79 CpG sites/33 genes from an independent gene set in maternal leukocyte DNA in PE and NL (n = 14 each) on an Illumina BeadChip platform. Replication performed on second cohort (PE = 12; NL = 32). Results: PE demonstrates differential methylation in POMC, AGT, CALCA, and DDAH1 compared with NL. Conclusion: Differential methylation in four genes associated with PE may represent a potential biomarker or an epigenetic pathophysiologic mechanism altering gene transcription.
Acknowledgments
We thank the Mayo Clinic Center for Individualized Medicine for providing access to the Biobank samples.
Declaration of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper.
Funding
1. Mayo Clinic: Mary Kathryn and Michael B. Panitch Career Development Award 2. Vesna D. Garovic (K08 HD051714) from the Eunice Kennedy Shriver National Institute of Child Health and Human Development. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Eunice Kennedy Shriver National Institute of Child Health and Human Development or the National Institutes of Health.3. This project was supported in part by funding from the Mayo Clinic Center for Individualized Medicine (CIM) Epigenomics Translational Program.
Additional information
Funding
Notes on contributors
Wendy M. White
Wendy M. White designed the study, carried out the methylation experiments, and drafted the manuscript; Zhifu Sun performed bioinformatics and statistical analysis; Kristi S. Borowski, Brian C. Brost, Norman P. Davies, and Carl H. Rose participated in design and coordination of the study; Vesna D. Garovic conceived the study, participated in design and coordination, and helped in drafting the manuscript. All authors read and approved the final manuscript.
Zhifu Sun
Wendy M. White designed the study, carried out the methylation experiments, and drafted the manuscript; Zhifu Sun performed bioinformatics and statistical analysis; Kristi S. Borowski, Brian C. Brost, Norman P. Davies, and Carl H. Rose participated in design and coordination of the study; Vesna D. Garovic conceived the study, participated in design and coordination, and helped in drafting the manuscript. All authors read and approved the final manuscript.
Kristi S. Borowski
Wendy M. White designed the study, carried out the methylation experiments, and drafted the manuscript; Zhifu Sun performed bioinformatics and statistical analysis; Kristi S. Borowski, Brian C. Brost, Norman P. Davies, and Carl H. Rose participated in design and coordination of the study; Vesna D. Garovic conceived the study, participated in design and coordination, and helped in drafting the manuscript. All authors read and approved the final manuscript.
Brian C. Brost
Wendy M. White designed the study, carried out the methylation experiments, and drafted the manuscript; Zhifu Sun performed bioinformatics and statistical analysis; Kristi S. Borowski, Brian C. Brost, Norman P. Davies, and Carl H. Rose participated in design and coordination of the study; Vesna D. Garovic conceived the study, participated in design and coordination, and helped in drafting the manuscript. All authors read and approved the final manuscript.
Norman P. Davies
Wendy M. White designed the study, carried out the methylation experiments, and drafted the manuscript; Zhifu Sun performed bioinformatics and statistical analysis; Kristi S. Borowski, Brian C. Brost, Norman P. Davies, and Carl H. Rose participated in design and coordination of the study; Vesna D. Garovic conceived the study, participated in design and coordination, and helped in drafting the manuscript. All authors read and approved the final manuscript.
Carl H. Rose
Wendy M. White designed the study, carried out the methylation experiments, and drafted the manuscript; Zhifu Sun performed bioinformatics and statistical analysis; Kristi S. Borowski, Brian C. Brost, Norman P. Davies, and Carl H. Rose participated in design and coordination of the study; Vesna D. Garovic conceived the study, participated in design and coordination, and helped in drafting the manuscript. All authors read and approved the final manuscript.
Vesna D. Garovic
Wendy M. White designed the study, carried out the methylation experiments, and drafted the manuscript; Zhifu Sun performed bioinformatics and statistical analysis; Kristi S. Borowski, Brian C. Brost, Norman P. Davies, and Carl H. Rose participated in design and coordination of the study; Vesna D. Garovic conceived the study, participated in design and coordination, and helped in drafting the manuscript. All authors read and approved the final manuscript.