Highly Sensitive Microplate-Elisa for Angiotensin I Using 3,3′,5,5′-Tetramethylbenzidine

F. Suzuki Department of Biotechnology Faculty of Agriculture, Gifu University, 501-11, Gifu, Japan

S. Yamashita Department of Biotechnology Faculty of Agriculture, Gifu University, 501-11, Gifu, Japan

A. Takahashi Department of Biotechnology Faculty of Agriculture, Gifu University, 501-11, Gifu, Japan

M. Ito Department of Biotechnology Faculty of Agriculture, Gifu University, 501-11, Gifu, Japan

S. Miyazaki Department of Internal Medicine, Gifu University, School of Medicine, 500, Gifu, Japan

Y. Nagata Department of Biotechnology Faculty of Agriculture, Gifu University, 501-11, Gifu, Japan

Y. Nakamura Department of Biotechnology Faculty of Agriculture, Gifu University, 501-11, Gifu, Japan

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Abstract

A microplate-ELISA was established for angiotensin (Ang) I using Ang I -peroxidase conjugate, antiAng I antibodies which could distinguish Ang I from Ang II and Ang III, and 3,3′,5,5′-tetramethylbenzidine. Ang I could be quantified in amounts as low as 1 pg by the spectrophotometric method. The present assay system was the most sensitive and rapid in all Ang I assay systems described so far. The assay system was applicable to determination of Ang I concentration in human plasma and abdominal fluid.

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Highly Sensitive Microplate-Elisa for Angiotensin I Using 3,3′,5,5′-Tetramethylbenzidine

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Highly Sensitive Microplate-Elisa for Angiotensin I Using 3,3′,5,5′-Tetramethylbenzidine

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