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Free Radical Research Volume 46, 2012 - Issue 9
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Research Article

Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging

Fei ShiGraduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P. R. China;The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing, P. R. China
,
Ben NieThe Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing, P. R. China;Department of Pharmacy, Wenzhou Medical College, University-town, Wenzhou, Zhejiang, P.R.China
,
Wei GanGraduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P. R. China;The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing, P. R. China
,
Xiao-Yang ZhouThe Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing, P. R. China
,
Yasumitsu TakagiFrontier Research Center, Fukuoka Dental College, Fukuoka, Japan
,
Hiroshi HayakawaFrontier Research Center, Fukuoka Dental College, Fukuoka, Japan
,
Mutsuo SekiguchiFrontier Research Center, Fukuoka Dental College, Fukuoka, Japan
&
Jian-Ping CaiThe Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, Beijing, P. R. ChinaCorrespondence[email protected]
 show all
Pages 1093-1098 | Received 20 Jan 2012, Accepted 25 Apr 2012, Published online: 21 May 2012
 
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Abstract

The levels of the oxidised forms of guanosine in leukocytes, plasma and urine of Macaca mulatta were determined using a sensitive method based on high-performance liquid chromatography–triple quadruple mass spectrometry (LC–MS/MS). The amounts of 8-oxo-7,8-dihydrodeoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosin (8-oxoGsn), derived from DNA and RNA, respectively, increased with age in leukocytes. The measurement of the free forms of oxidised guanosine revealed similar age-dependent increases of 8-oxo-dGsn and 8-oxoGsn in both plasma and urine, which showed considerably larger amounts of 8-oxoGsn than 8-oxo-dGsn. The 8-oxoGsn content of urine could be a useful biomarker for evaluating aging, as age-dependent increases of 8-oxoGsn are more evident in urine compared to plasma and because urine samples are readily available.

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