128
Views
4
CrossRef citations to date
0
Altmetric
Research Articles

GANRA-5 protects both cultured cells and mice from various radiation types by functioning as a free radical scavenger

, , , , , & show all
Pages 670-678 | Received 25 Aug 2013, Accepted 24 Feb 2014, Published online: 25 Mar 2014
 

Abstract

The radio-protective effects of the oxazolone derivative chemical compound 4-(4-methoxy-3-methoxyphenyl-methyl)-2-phenyl- 5(4H)-oxazolone (GANRA-5) against different types of radiation including X-rays, carbon ion beams, microwaves and ultraviolet light (UV) were studied. Cell proliferation/cytotoxicity assay and colony-forming assay were conducted to evaluate the toxicity of GANRA-5. To test its influence on the induction of double-stranded break (DSB) formation and genomic instability, γH2AX focus-forming assay as well as cytokinesis-block micronucleus assay was utilized. Our results indicate that GANRA-5 exhibits low toxicity, while providing high radio-protective effects for MRC-5 cells against different types of radiation. We also found that GANRA-5 acts as a free radical scavenger. Our animal studies provided evidence that GANRA-5 significantly increases the survival rate of mice after X-ray irradiation. Analyses of hemogram, visceral index and detection of superoxide dismutase (SOD) and malondialdehyde (MDA) in the viscera indicate both low toxicity of GANRA-5, combined with its ability to shield radiation risk. In conclusion, our results suggest that GANRA-5 has the potential to be used as a safe and efficient radio-protectant.

Acknowledgements

The authors thank Drs. Yanan Zhang and Jianghua Wan for their assistance with the experiment procedures. We are grateful to the HIRFL-CSR crew for providing excellent technical assistance with the operation of the heavy ion beams.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.

This work was supported by grants of the Major State Basic Research Development Program of China (973 Program, No. 2010CB834201), the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA01040411), and the National Natural Science Foundations of China awarded to Guangming Zhou (No. 11335011, 31370846 and 10979062) and Jufang Wang (No. U1232125 & 31270895).

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 61.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 940.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.