Gossypetin ameliorates ionizing radiation-induced oxidative stress in mice liver—a molecular approach

Abstract

Radioprotective action of gossypetin (GTIN) against gamma (γ)-radiation-induced oxidative stress in liver was explored in the present article. Our main aim was to evaluate the protective efficacy of GTIN against radiation-induced alteration of liver in murine system. To evaluate the effect of GTIN, it was orally administered to mice at a dose of 30 mg/kg body weight for three consecutive days prior to γ-radiation at a dose of 5 Gy. Radioprotective efficacy of GTIN were evaluated at physiological, cellular, and molecular level using biochemical analysis, comet assay, flow cytometry, histopathology, immunofluorescence, and immunoblotting techniques. Ionizing radiation was responsible for augmentation of hepatic oxidative stress in terms of lipid peroxidation and depletion of endogenous antioxidant enzymes. Immunoblotting and immunofluorescence studies showed that irradiation enhanced the nuclear translocation of nuclear factor kappa B (NF-κB) level, which leads to hepatic inflammation. To investigate further, we found that radiation induced the activation of stress-activated protein kinase/c-Jun NH2-terminal kinase (SAPK/JNK)-mediated apoptotic pathway and deactivation of the NF-E2-related factor 2 (Nrf2)-mediated redox signaling pathway, whereas GTIN pretreatment ameliorated these radiation-mediated effects. This is the novel report where GTIN rationally validated the molecular mechanism in terms of the modulation of cellular signaling system’ instead of ‘ This is the novel report where GTIN is rationally validated in molecular terms to establish it as promising radioprotective agents. This might be fruitful especially for nuclear workers and defense personnel assuming the possibility of radiation exposure.

Keywords::

• gossypetin
• gamma radiation
• antioxidant
• inflammation
• lipid peroxidation
• cell signaling

Acknowledgment

The authors are thankful to Professor. M. Nandi of SINP, Kolkata for providing necessary animal irradiation facilities. SD acknowledges CSIR, GOI, for fellowship to KM, WB DBT, Kolkata, India for fellowship support to DD, CPEPA, UGC for fellowship to SBK & AS, UGC-DAE to MS, and LSRB to UD. The authors acknowledge Dr Debasish Bandhopadhyay of CGCRI, Kolkata for his editorial comments on manuscripts.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Supplementary material available online

Supplementary Figure 1 to be found online at http://informahealthcare.com/doi/abs/10.3109/10715762.2015.1053878.