On the role of histamine receptors in regulating pigmentary responses in Oreochromis mossambicus melanophores

Abstract

Purpose: The present work was carried out to reveal the involvement of histamine receptors at the neuro-melanophore junction of teleost, Oreochromis mossambicus.

Methods: The isolated scale melanophores were assayed using the mean melanophore size index and their responses were recorded in presence of various concentrations of histamine along with H₁ and H₂ receptor specific agonists and antagonist and potentiator compound 48/80.

Results: Melanophores showed high sensitivity to histamine and its specific agonists. Histamine caused a dose-dependent pigment aggregation, whereas 2-(2-Pyridyl) ethylamine (PEA), a specific H₁R agonist also caused aggregation in a similar manner. Conversely, amthamine, a specific H₂R agonist resulted in pigment dispersion. The effects were antagonized by mepyramine; specific H₁R antagonist and ranitidine a specific H₂R antagonist.

Conclusion: It is concluded that O. mossambicus melanophores have both H₁ and H₂ receptors which mediate melanophore aggregation and dispersion respectively. Compound 48/80 augmented the melanin-aggregating and dispersing effects of PEA and amthamine. It is suggested that the effect of histamine is directly mediated through H1 and H2 receptors, whereas H1Rs may be predominantly involved in the aggregatory responses.

Keywords::

• Melanophores
• pigment translocation
• histamine
• histamine receptors

Acknowledgment

We thank Principal and Secretary Saifia College of Science for providing necessary facilities for carrying out the study. Thanks are due to Dr. Rob Leurs, Center for Drug Research Leiden/Amsterdam for providing generous gifts of amthamine and ranitidine and Dr. Samreen Arshad, Sanofi-Aventis, Bridgewater, NJ, USA for a generous gift of mepyramine maleate.

Declaration of interest

The authors report no conflicts of interest.