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Research Article

Variations in biochemical and histological characteristics of WSSV infected green tiger shrimp Penaeus semisulcatus

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Pages 386-395 | Received 27 Jan 2014, Accepted 12 Mar 2014, Published online: 16 Apr 2014
 

Abstract

White Spot Syndrome Virus causes viral disease in crustaceans and generates a significant burden in the developing nations. Biochemical and immunological assays were performed in WSSV infected Penaeus semisulcatus which were monitored in different salinity conditions. Continuous exposure of shrimps to WSSV showed a reduced life span, indicating the pathogenicity in Penaeidae species. Hence, this study is intended to investigate the protective antioxidant potential of the innate immune system consisting biochemical and morphological alterations. Penaeus semisulcatus challenged with white spot syndrome virus (5.5 × 104 copy number; WSSV) reared at different salinity 5, 15, 25 (control) and 35 g/L were examined after 0–120 h for immunological parameters such as total hemocyte count (THC), phenoloxidase (PO) and respiratory burst (RB) and alkaline and acid phosphatase activities. After 72 h, the WSSV injected P. semisulcatus tissues were histopathologically sectioned and stained. This study would be helpful to understand host–pathogen interaction and envisages the improvement of better management practices in shrimp aquaculture system.

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