Comparison of plasma from healthy nonsmokers, smokers, and lung cancer patients: Pattern-based differentiation profiling of low molecular weight proteins and peptides by magnetic bead technology with MALDI-TOF MS

Abstract

Context: Smoking is the major contributor of lung cancer (LC), which accounts for millions of death.

Objective: This study focused on the correlation between the proteomic profiling of LC patients, and healthy nonsmokers and smokers.

Method: Pattern-based peptide profiling of 186 plasma samples was performed through reversed-phase chromatography-18 magnetic bead fractionation coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and resulted data were evaluated statistically by ClinProTool.

Results: Marker peaks at m/z 1760, 5773, 5851, 2940, and 7172 were found with an excellent statistical figure.

Conclusion: Selected marker peaks can be served as a differentiated tool of LC patients with high sensitivity and specificity.

Keywords::

• C-18 magnetic bead
• ClinProTools software
• MALDI-TOF-MS
• smokers
• lung cancer
• plasma peptide
• pattern-based profiling

Acknowledgments

Thanks to the staff of JPMC and Diagnostic laboratory (PCMD) for their assistance in blood collection. Thanks to all volunteers involved in this study and Prof. Dr. Daniel Hoessli for reading this article and for providing useful suggestions. Thanks to Mr. Arslan Ali, Mr. Safdar, Miss Amna Jabbar, Miss Shulmaila Mazhar, and Miss Mahwish Saleem (ICCBS) for their assistance in statistical analysis.

Declaration of interest

The authors report no conflict of interest.