DNA microarray study of genes differentiating acute myocardial infarction patients from healthy persons

Abstract

Objective: Using oligonucleotide microarrays HG-U133A, we here studied the expression levels of genes that could differentiate between patients with myocardial infarction (MI) from healthy subjects, as well as to select among such genes those that seem crucial for manifestation of cardiovascular diseases.

Materials and methods: The microarray study was conducted using material derived from blood samples collected in 17 individuals.

Results: Analysis of gene expression data from 17 microarrays allowed identification of 28 genes strongly differentiating the examined groups.

Conclusion: The differentiating genes that we tracked down indicate possible linkage with atherosclerotis and could be a prognostic marker for development of cardiovascular diseases.

Keywords::

• Atherosclerosis
• cardiovascular diseases
• oligonucleotide microarrays

Acknowledgments

Molecular work was performed in Department of Molecular Biology, School of Pharmacy and Division of Laboratory Medicine, Medical University of Silesia in Katowice.

Declaration of interest

The research project entitled “Oligonucleotide microarray study of proinflammatory genes expression profile in mononuclear peripheral blood cells and right atrium cells obtained from atherosclerotic and ACS (unstable coronary disease, myocardial infarction) patients” and which was designed according to Declaration of Helsinki-based GCP guidelines was positively endorsed by the Bioethics Commision of the Medical University of Silesia in Katowice on December 21, 2004 (resolution NN-6501-223/I/04).