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Abstract
Context: The study of DNA base and nucleotide excision repair gene polymorphisms in bladder cancer seems to have a predictive value because of the evident relationship between the DNA damage response induced by environmental mutagens and cancer predisposition.
Objective: The objective was to determine OGG1 Ser326Cys, XRCC1 Arg399Gln, XPD Asp312Asn, and ERCC6 Met1097Val polymorphisms in bladder cancer patients as compared to controls.
Methods: Both groups were predominantly represented by Belarusians and Eastern Slavs. DNA samples from 336 patients and 370 controls were genotyped using a PCR-RFLP method.
Results: The genotype distributions were in agreement with the Hardy–Weinberg equilibrium. The minor allele frequencies in the control population were in the range of those in Caucasians in contrast to Asians. The OGG1 326 Ser/Cys and XPD 312 Asp/Asn heterozygous genotypes were inversely associated with cancer risk (OR [95% CI] = 0.69 [0.50–0.95] and 1.35 [1.0–1.82], respectively). The contrasting effects of these genotypes were potentiated due to their interactions with smoking habit or age.
Conclusions: Among four DNA repair gene polymorphisms, the OGG1 326 Ser/Cys and XPD 312 Asp/Asn heterozygous genotypes might be recognized as potential genetic markers modifying susceptibility to bladder cancer in Belarus.
Acknowledgements
The authors are sincerely grateful to Professor Irena Szumiel (Centre for Radiobiology & Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Poland) and Professor Aksana Kucher (Laboratory of Molecular Genetics, Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences, Russia) for previous critical reading of the manuscript, discussion of the results and helpful comments.