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Biomarkers Volume 19, 2014 - Issue 7
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Technical Brief

A novel cost-effective assay based on real-time PCR for COMT Val158Met genotyping

Diego A. OjedaLaboratory of NeuroPsychiatric Genetics, Biomedical Sciences Research Group, School of Medicine, Universidad Antonio Nariño BogotáColombia;School of Sciences, Universidad Antonio Nariño BogotáColombiaCorrespondence[email protected]
,
Sandra López-LeónNovartis Pharmaceuticals Corporation East Hanover, NJUSA
&
Diego A. ForeroLaboratory of NeuroPsychiatric Genetics, Biomedical Sciences Research Group, School of Medicine, Universidad Antonio Nariño BogotáColombia
Pages 567-570 | Received 25 Jun 2014, Accepted 21 Jul 2014, Published online: 06 Aug 2014
 
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Abstract

A functional polymorphism in the catechol-O-methyltransferase (COMT) gene (Val158Met) has been associated with a large number of human diseases and endophenotypes. The aim of this study was to develop a novel cost-effective assay to genotype this polymorphism. The novel assay was based on the combination of allele-specific PCR and high-resolution melting in a qPCR platform. Melt-curve analysis allowed a clear differentiation of the three genotypes. This novel assay could be implemented in the study of a large number of diseases and endophenotypes related to COMT dysfunction and could be extended for the analysis of other functional polymorphisms.

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