Direct saliva transcript analysis as a novel non-invasive method for oestrus marker detection in buffaloes

Abstract

Salivary RNA-based biomarkers are not available for any physiological condition in farm animals. Hence, an objective of this study was to perform salivary transcript analysis in buffaloes. Saliva, after removal of the cells and particulate matter, was directly used for RT-PCR without RNA isolation. Direct saliva transcript analysis (DSTA) showed a suggestively significant higher expression of the Heat shock protein 70 (HSP70) and Toll-like receptor 4 (TLR4) at oestrus than the diestrous period in buffaloes by a non-parametric Mann-Whitney U test. Therefore, DSTA without RNA isolation is an easy method to identify salivary RNA markers for oestrus detection in buffaloes.

• HSP70
• salivary-RNA
• TLR4

Acknowledgements

The authors are thankful to Mr. Shankar, an animal handler for handling buffaloes during the sample collection.

Declaration of interest

The authors have no financial and non-financial competing interests with other people and organisations, influencing the paper content. The authors are thankful to the Director, NDRI, Karnal, India; NASF, ICAR, for their financial assistance.