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Biomarkers Volume 21, 2016 - Issue 3
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A modified and cost-effective method for hair cortisol analysis

Lianbin XiangLaboratory of Behavioral Immunology Research, Division of Clinical Immunology and Allergy, Department of Medicine, Center of Biostatistics and Bioinformatics, University of Mississippi Medical Center, Jackson, MS, USACorrespondence[email protected]
,
Imran SunesaraLaboratory of Behavioral Immunology Research, Division of Clinical Immunology and Allergy, Department of Medicine, Center of Biostatistics and Bioinformatics, University of Mississippi Medical Center, Jackson, MS, USA
,
Kristina E. RehmLaboratory of Behavioral Immunology Research, Division of Clinical Immunology and Allergy, Department of Medicine, Center of Biostatistics and Bioinformatics, University of Mississippi Medical Center, Jackson, MS, USA
&
Gailen D. Marshall JrLaboratory of Behavioral Immunology Research, Division of Clinical Immunology and Allergy, Department of Medicine, Center of Biostatistics and Bioinformatics, University of Mississippi Medical Center, Jackson, MS, USA
Pages 200-203 | Received 12 May 2015, Accepted 05 Dec 2015, Published online: 15 Jan 2016
 
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Abstract

Hair cortisol may hold potential as a biomarker for assessment of chronic psychological stress. We report a modified and cost-effective method to prepare hair samples for cortisol assay. Hair samples were ground using an inexpensive ball grinder – ULTRA-TURRAX tube drive. Cortisol was extracted from the powder under various defined conditions. The data showed that the optimal conditions for this method include cortisol extraction at room temperature and evaporation using a stream of room air. These findings should allow more widespread research using economical technology to validate the utility of hair cortisol as a biomarker for assessing chronic stress status.

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