Increase in urinary markers during the acute phase reflects the degree of chronic tubulointerstitial injury after ischemia-reperfusion renal injury

Abstract

Context: Acute kidney injury (AKI) could lead to progressive chronic kidney disease (CKD). Objectives: To demonstrate that urinary markers in AKI are associated with the degree of persistent renal injury. Material and methods: Human L-FABP chromosomal transgenic (T_g) mice were subjected to ischemia-reperfusion (I/R) clamping renal pedicle for 20 min or 30 min. Kidneys were obtained at one and 40 days after I/R. Results: Urinary L-FABP, NGAL, Kim-1 and albumin levels increased during the acute phase and were significantly correlated with the degree of tubulointerstitial fibrosis during the chronic phase. Discussion and conclusion: These markers could detect higher risk of progression to CKD.

Keywords:

• Acute kidney injury
• AKI to CKD transition
• chronic kidney disease
• renal damage

Acknowledgments

We thank Ms. Kimie Katayama and Ms. Jyunko Asano for their technical assistance.

Disclosure statement

T. Sugaya is the Director and Senior Scientist of CMIC HOLDINGS Co., Ltd., the company that produced the kits for L-FABP analysis. None of the other authors have conflicts of interest or financial disclosures of any relevance to the present study.

Funding information

This work was supported by the Naito Foundation, Takeda Science Foundation, and Research Fund of Mitsukoshi Health and Welfare Foundation.

Author contributions

M. Hisamichi, A. Kamijo-Ikemori and Y. Shibagaki, conception and design of research; M. Hisamichi, A. Kamijo-Ikemori, D. Ichikawa, and S. Hoshino performed experiments; M. Hisamichi and A. Kamijo-Ikemori analyzed data; M. Hisamichi, A. Kamijo-Ikemori, D. Ichikawa, T. Sugaya, K Hirata, K. Kimura and Y. Shibagaki interpreted results of experiments; T. Sugaya, K Hirata, K. Kimura and Y. Shibagaki advised them critically for important intellectual content; M. Hisamichi prepared figures; M. Hisamichi and A. Kamijo-Ikemori drafted manuscript; A. Kamijo-Ikemori finally approved the version to be published.