The nucleotide hydrolysis is altered in blood serum of streptozotocin-induced diabetic rats

Abstract

Ectonucleotidases and the nucleotide metabolism have been implicated as important regulators of various tissue functions in diabetes disease. Here we evaluated the ectonucleotidase activities and the profile of extracellular ATP metabolism in blood serum of streptozotocin (STZ)-induced diabetic rats. We observed a raise in ATP, ADP, AMP, and 5′-TMP hydrolysis in blood serum after 30 days of diabetes induction, when compared with the citrate group. However, in serum of rats treated 6 days with insulin, the hydrolysis returned to the control levels. Extracellular ATP metabolism estimated by HPLC analysis showed a rapid hydrolysis of extracellular ATP by diabetic animals, leading to the formation of high levels of adenosine when compared with citrate and insulin groups. Since in diabetes the vascular disease is frequently present, the alterations observed are important, because these enzymes control the nucleotides/nucleosides ratio in the circulation and thus the events related to haemostasis.

Keywords::

• E-NTPDases
• ecto-5′-nucleotidase/CD73
• E-NPPs
• diabetes

Acknowledgements

This work was supported by grants and fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Centro Universitário Metodista do IPA.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.