A combination of two electrophoretical approaches for detailed proteome-based characterization of SCLC subtypes

Abstract

Context: Small cell lung cancers (SCLC) are heterogeneous and tumours differ in growth characteristics and treatment resistance. Objective: To get insight into the underlying protein profiles responsible for this heterogeneity, two subtypes of SCLC cells mutually differing in chemo resistance properties and growth characteristics are analysed. Materials and methods: Two different electrophoresis approaches in combination with mass spectrometry were used to detect differences between the SCLC cell lines GLC1 and GLC1M13: IEF/SDS-PAGE as well as cetyltrimethylammonium bromide (CTAB)-SDS-PAGE. Results: Altogether 60 non redundant differentially expressed proteins were found of which 5 were verified by Western Blot analysis. Discussion: Most of these proteins identified are involved in processes of tumour progression. Therefore, these proteins are interesting candidates for further functional analysis. Conclusion: Additional CTAB-SDS page is a complementary method to IEF-SDS page revealing a complete new subset of proteins differentially expressed between GLC1 and GLC1 M13 cells SCLC subtypes.

• Cancer
• cells
• chemoresistance
• DIGE
• lung
• SCLC

Acknowledgements

The authors thank Kathy Pfeiffer for excellent technical assistance.