Abstract
Adipose tissue and skeletal muscle are organs that respond strongly to obesity and physical activity exhibiting high secretory activity. To identify novel putative adipomyokines, comparative expression studies of skeletal muscle and adipose tissue of lean (C57BL/6J) and obese (C57BL/6J on a high-fat diet and NZO) mice, of sedentary and endurance trained C57BL/6J mice and of cattle characterized by different amounts of intramuscular fat were combined with human secretome data and scored. In highly regulated transcripts, we identified 119 myokines, 79 adipokines and 22 adipomyokines. Network analysis of these candidates revealed remodelling of extracellular matrix and tissue fibrosis as relevant functions of several of these candidates. Given the pathophysiogical relevance of fibrosis for adipose-muscle-cross-talk in obesity and type 2 diabetes and its physiological role in exercise adaptation and meat quality of farm animals, they represent interesting candidates for further investigations in different research areas and species.
Acknowledgements
This work was supported by a grant of the Leibniz Association (grant no. SAW-2013-FBN-3), the State of Brandenburg, the State of Nordrhein-Westfalen (Ministry of Science and Research of the State of North Rhine-Westphalia), by the German Federal Ministry of Education and Research (BMBF) (grant 01GI0925 for the German Centre for Diabetes Research DZD e.V.) and the German Federal Ministry of Health. We wish to thank Elke Schwitulla, Kerstin Jahnke, Annette Jugert, Hanni Tychsen and Ines Rothe for expert technical assistance as well as Mario Ost for providing the microarray data of the UCP1 Tg mouse model.
Declaration of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.
Supplementary material available online