Effect of Rat Serum on Glycerol Metabolism in Adipose Tissue in Vitro

Abstract

It has been proposed that the lipolytic effect of serum is based on the presence of either lipoproteins or catecholamines. To test these hypotheses, pieces of epididymal fat pads from fed rats were incubated in the presence of albumin and glucose for 120 min. The addition of rat serum (5 μl/vial) enhanced the rates of both glycerol release to the media and [U-¹⁴C] glycerol utilization by the tissue. Heparin did not alter these parameters or the response produced by serum. VLDL from rat plasma also enhanced glycerol release and utilization for the formation of CO₂ and lipids, and heparin significantly augmented these effects. Neither of the conditions studied affected the percentual distribution of ¹⁴C-lipid fractions in the tissues. It is known that in similar conditions to those used in the present study, adrenaline produces a decrease in the utilization of glycerol. Thus our findings do not support the proposed hypothesis explaining the fat-mobilizing action of serum, the mechanism of which remains to be determined.