622
Views
8
CrossRef citations to date
0
Altmetric
Orginal Article

Epigenetic markers for newborn congenital heart defect (CHD)

, , , , , & show all
Pages 1881-1887 | Received 22 Aug 2014, Accepted 02 Jul 2015, Published online: 01 Oct 2015
 

Abstract

Objective: Our objective was to determine whether there were significant differences in genome-wide DNA methylation in newborns with major congenital heart defect (CHD) compared to controls. We also evaluated methylation of cytosines in CpG motifs for the detection of these CHDs.

Methods: Genome-wide DNA methylation analysis was performed on DNA from 60 newborns with various CHDs, including hypoplastic left heart syndrome, ventricular septal deficit, atrial septal defect, pulmonary stenosis, coarctation of the aorta and Tetralogy of Fallot, and 32 controls.

Results: Highly significant differences in cytosine methylation were seen in a large number of genes throughout the genome for all CHD categories. Gene ontology analysis of CHD overall indicated over-represented biological processes involving cell development and differentiation, and anatomical structure morphogenesis. Methylation of individual cytosines in CpG motifs had high diagnostic accuracy for the detection of CHD. For example, for coarctation one predictive model based on levels of particular cytosine nucleotides achieved a sensitivity of 100% and specificity of 93.8% (AUC = 0.974, p < 0.00001).

Conclusion: Profound differences in cytosine methylation were observed in hundreds of genes in newborns with different types of CHD. There appears to be the potential for development of accurate genetic biomarkers for CHD detection in newborns.

Acknowledgement

We thank the Michigan Department of Community Health which supplied the blood specimens.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.

Web resources

The URLs for methods and databases presented herein are as follows:

Illumina: http://www.illumina.com/

Genome Studio: http://www.solexa.com/gsp/genomestudio_software.ilmn

Ensemble: http://www.ensembl.org/

UCSC: http://genome.ucsc.edu/

NCBI: http://www.ncbi.nih.gov/

DAVID/EASE: http://david.abcc.ncifcrf.gov/

Web Gestalt: http://genereg.ornl.gov/webgestalt/

Chilibot: www.chilibot.net

Ingenuity Systems: www.ingenuity.com

Online Mendelian Inheritance in Man (OMIM): http://www.ncbi.nlm.nih.gov/Omim/

Supplementary material available online

Log in via your institution

Log in to Taylor & Francis Online

PDF download + Online access
  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 65.00 Add to cart
* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.