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Toxicology Mechanisms and Methods Volume 22, 2012 - Issue 6
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Research Article

Tyrosine hydroxylase assay: a bioassay for aryl hydrocarbon receptor-active compounds based on tyrosine hydroxylase promoter activation

Eiichi AkahoshiFrontier Research Laboratory, Corporate Research and Development Center, Toshiba Corporation, Komukai-Toshiba-cho, Saiwai-ku, Kawasaki, Japan
,
Seiko YoshimuraFrontier Research Laboratory, Corporate Research and Development Center, Toshiba Corporation, Komukai-Toshiba-cho, Saiwai-ku, Kawasaki, Japan
,
Saeko UrunoFrontier Research Laboratory, Corporate Research and Development Center, Toshiba Corporation, Komukai-Toshiba-cho, Saiwai-ku, Kawasaki, Japan
,
Satoshi ItohAdvanced Institute for Computational Science, RIKEN, Minatojima-minami-machi, Chuo-ku, Kobe, Japan
&
Mitsuko Ishihara-SuganoFrontier Research Laboratory, Corporate Research and Development Center, Toshiba Corporation, Komukai-Toshiba-cho, Saiwai-ku, Kawasaki, JapanCorrespondence[email protected]
Pages 458-460 | Received 20 Dec 2011, Accepted 17 Feb 2012, Published online: 22 Mar 2012
 
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Abstract

Tyrosine hydroxylase (TH) assay is a novel bioassay for screening aryl hydrocarbon receptor (AhR)-active compounds with diverse toxicities. The TH assay employs reporter genes to evaluate TH promoter activity, which is linked to dopamine synthesis and regulated by the AhR-aryl hydrocarbon receptor responsive element-III (AHRE-III)-mediated pathway. The toxic equivalency factors for higher chlorinated dioxin congeners in the TH assay (TH-TEF) exhibit the same tendency as those for the WHO-TEF, indicating that the activity of the TH assay is consistent with that of existing methods. Furthermore, other chemicals, such as lower chlorinated dioxin congeners that were minimally investigated in the past could be detected preferentially by this assay.

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