Deoxynivalenol induces cytotoxicity and genotoxicity in animal primary cell culture

Abstract

Deoxynivalenol (DON), a mycotoxin produced by Fusarium graminearum, is widely found as a contaminant of food. DON is responsible for a wide range of toxic activities, including gastro-intestinal, lymphoid, bone-marrow and cardiotoxicity. But, the complete explorations of toxicity in terms of hepatotoxicity, nephrotoxicity, cytotoxicity and genotoxicity as well have not been documented well. Again, the mechanisms through which DON damages the DNA and promotes cellular toxicity are not well established. Considering the above fact, this research article is focused on the effects of DON-induced toxicities on experimental animal model as well as its effects on cellular level via various toxicological investigations. DON treatment showed cytotoxicity and DNA damage. Further, flow cytometric analysis of hepatocytes showed cellular apoptosis, suggesting that DON-induced hepatotoxicity is, may be partly, mediated by apoptosis. Moreover, significant differences were found in each haematology and clinical chemistry value, either (p > 0.05). No abnormality of any organ was found during histopathological examination. Hence, it can be concluded that DON induces oxidative DNA damage and increases the formation of centromere positive micronuclei due to aneugenic activity.

• Acute oral toxicity
• cytotoxicity
• deoxynivalenol
• genotoxicity
• mycotoxin

Acknowledgements

Shweta Singh is grateful to Defence Research Laboratory, Tezpur, Assam, India for providing necessary instrumental facilities for this research work and as well as to the administration of the Gauhati University, Gauhati, Assam, India for providing necessary administrative support for carrying out her Ph.D work.

Declaration of interest

The authors declare that they have no conflicts of interest to declare in connection with the contents of this manuscript.