In Vitro Percutaneous Absorption Experiments: A Guide to the Technique for Use in Toxicology Assessments

Summary

Assessments of percutaneous absorption using a variety of in vitro techniques are now commonplace in many laboratories. A major advantage of this technique is the ability to estimate the absorption of toxic chemicals through human skin without putting human subjects at risk. This article discusses the essential features of the in vitro percutaneous absorption method. Specifically, the design of diffusion cells, choice of suitable receptor fluids, preparation of skin membranes, and the effect of storage and temperature in these experiments are discussed. Literature references that indicate that in vitro assessments of percutaneous absorption can predict in vivo absorption are briefly discussed.

Key Words: :

• Percutaneous absorption
• In vitro
• Toxicology assessment