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Abstract
The study examined whether pregastrulation rat embryos can be used as a model for demonstrating in vivo the toxic and teratogenic effects of all-trans retinoic acid (tRA). The tRA was administered per os to Wistar rats on day 6 of gestation (the presence of sperm was taken as day 1) in a single dose of 120 mg/kg body weight. In the litters examined on days 9 and 14 of gestation, tRA significantly increased the number of resorbed embryos. Examination on day 14 of pregnancy revealed that tRA also caused a high proportion of embryos to be growth-retarded (with CR length at least 20% less than that of controls). Growth retardation was found in 6 of 7 tested litters. Both external and skeletal malformations were observed in 20 day fetuses when tRA was administered on day 6 of gestation. Rats receiving tRA on days 11 or 14 of gestation served as positive controls, and also showed malformations. These results indicate that pregastrulation rat embryo can be used for assessing the pathways contributing to the teratogenic effect of tRA.
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