Non-invasive determination of cellular oxygen consumption as novel cytotoxicity assay for nanomaterials

Abstract

Investigating the safety of nanoparticles is essential for many fields of their applications, in particular for consumer products, food and medicines. The conventional dye and fluorescence-based cytotoxicity assays are limited by the interference of such readouts with nanomaterials. This holds in particular when nanomaterials have been fluorescently labelled for other purposes, for example, confocal microscopy. Moreover, most of these assays are invasive, that is, typically involve irreversible changes or destruction of cells and hence only allowing one endpoint measurement. Therefore, a non-invasive method for the detection of cytotoxicity was developed which is based on the automated online monitoring of the oxygen concentration in solution (SensorDish® Reader). Fluorescently labelled silica nanoparticles with different sizes and surface modifications were used as model systems to explore this novel assay. Thereby, the SensorDish® Reader allows a life documentation of the cellular behaviour and clarifies that size, time, concentration and surface modification of nanoparticles affect cellular viability.

Keywords::

• cytotoxicity
• silica nanoparticles
• SensorDish® Reader
• non-invasive assay
• Caco-2 cells

Acknowledgements

This project was supported by the Federal German Ministry of Education and Research BMBF (NanoBiocomp, Förderkennzeichen 0312025F).