Impact of non-functionalized and amino-functionalized multiwall carbon nanotubes on pesticide uptake by lettuce (Lactuca sativa L.)

Abstract

The effect of non-functionalized and amino-functionalized multiwall carbon nanotube (CNT) exposure, as well as the impact of CNT presence on coexistent pesticide accumulation, was investigated in lettuce (Lactuca sativa L.). Lettuce seeds were sown directly into CNT-amended vermiculite (1000 mg L⁻¹) to monitor phytotoxicity during germination and growth. During growth, lettuce seedlings were subsequently exposed to chlordane (cis-chlordane [CS], trans-chlordane [TC] and trans-nonachlor [TN]) and p,p′-DDE (all at 100 ng/L) in the irrigation solution for a 19-d growth period. CNT exposure did not significantly influence seed germination (82–96%) or plant growth. Similarly, pesticide exposure had no impact on plant growth, total pigment production or tissue lipid peroxidation. After 19 d, the root content of total chlordane and p,p′-DDE was 390 and 73.8 µg g⁻¹, respectively; in plants not exposed to CNTs, the shoot levels were 1.58 and 0.40 µg g⁻¹, respectively. The presence and type of CNT significantly influenced pesticide availability to lettuce seedlings. Non-functionalized CNT decreased the root and shoot pesticide content by 88% and 78%, respectively, but amino-functionalized CNT effects were significantly more modest, with decreases of 57% in the roots and 23% in the shoots, respectively. The presence of humic acid completely reversed the reduced accumulation of pesticides induced by amino-functionalized CNT, likely due to strong competition over adsorption sites on the nanomaterial (NM). These findings have implications for food safety and for the use of engineered NMs in agriculture, especially with leafy vegetables.

• Lettuce
• NF-MWCNT
• NH₂-MWCNT
• pesticides
• uptake

Acknowledgements

The authors gratefully acknowledge T. Arsenault and K. Prapayotin-Riveros for technical assistance.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.

H. H. would like to deeply thank the Council for the International Exchange of Scholars (CIES) for supporting his travel and stay at the CAES, New Haven, CT, USA, under the Fulbright Visiting Scholar Program (Grant ID 68120784). The authors also acknowledge USDA AFRI grant 2011-67006-30181.